Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

830
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
830
Methods of Classification and Identification01:28

Methods of Classification and Identification

2.2K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of <i>Escherichia coli</i> isolates associated with bovine mastitis in Austria.

Frontiers in veterinary science·2026
Same author

Quantitative Multiplex Digital PCR with Fluorescence-Encoded Nanoreactor Beads.

Analytical chemistry·2026
Same author

Clonal structure, resistome and virulence gene profiles of methicillin-resistant <i>Staphylococcus aureus</i> from retail chicken meat in the United Arab Emirates.

Frontiers in microbiology·2026
Same author

Staphylococcus aureus sequence type 71 is a chimera that emerged twice.

BMC genomics·2026
Same author

Prevalence and genetic characterization of methicillin-resistant Staphylococcus aureus in Commercial aquaculture farms in Egypt.

Scientific reports·2026
Same author

Phage-antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance.

Journal of biomedical science·2026
Same journal

Ebola laboratory preparedness at frontline hospitals: can we or can't we?

Journal of clinical microbiology·2026
Same journal

Reporting macrolide-resistant <i>Mycoplasma pneumoniae</i>: a diagnostic obligation?

Journal of clinical microbiology·2026
Same journal

Diagnostic value of HHV-6A/B genotyping in immunocompromised adults.

Journal of clinical microbiology·2026
Same journal

Multicenter performance evaluation of the Simplexa <i>C. auris</i> Direct assay for the detection of <i>Candida auris</i> colonization in bilateral axilla/groin swabs.

Journal of clinical microbiology·2026
Same journal

Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

Journal of clinical microbiology·2026
Same journal

Risk assessment and mitigation of hepatitis C virus RNA carryover contamination in a reflex testing algorithm.

Journal of clinical microbiology·2026
See all related articles

Related Experiment Video

Updated: Apr 25, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

9.0K

DNA microarray-based typing of Streptococcus agalactiae isolates.

Heike Nitschke1, Peter Slickers2, Elke Müller2

  • 1Institute for Medical Microbiology and Hygiene, Technische Universität Dresden, Dresden, Germany Department of Obstetrics and Gynecology, Elblandklinikum Meißen, Meissen, Germany.

Journal of Clinical Microbiology
|August 29, 2014
PubMed
Summary
This summary is machine-generated.

A new microarray effectively types Streptococcus agalactiae (group B Streptococcus) by analyzing 11 key markers. This method reveals genetic diversity and helps understand the epidemiology of this common bacterium, which causes serious infections in newborns.

More Related Videos

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

11.4K
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
12:18

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

24.2K

Related Experiment Videos

Last Updated: Apr 25, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

9.0K
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

11.4K
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
12:18

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

24.2K

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Streptococcus agalactiae, or group B Streptococcus (GBS), is a common colonizer of the urogenital tract.
  • GBS is a leading cause of serious infections like septicemia, meningitis, and pneumonia in newborns.
  • Accurate typing methods are crucial for understanding GBS epidemiology and transmission.

Purpose of the Study:

  • To design and validate a microarray for typing Streptococcus agalactiae isolates.
  • To assess the relationship between traditional typing markers and multilocus sequence typing (MLST) defined sequence types.
  • To investigate the population structure and epidemiology of S. agalactiae using a novel array-based typing scheme.

Main Methods:

  • Development and validation of a microarray targeting virulence-associated markers and resistance genes in GBS.
  • Comparison of array-based typing results with multilocus sequence typing (MLST) for 448 S. agalactiae isolates.
  • Analysis of 11 selected markers (bac, alp, capsule locus, etc.) for strain differentiation.

Main Results:

  • Individual typing markers (e.g., capsule type, alp allele) varied independently and did not reliably predict MLST sequence types, suggesting frequent genomic recombination.
  • The defined set of 11 markers successfully differentiated the 448 isolates into 76 strains, largely clustering according to MLST-defined clonal complexes.
  • Evidence of region- and host-specific differences in S. agalactiae population structure was observed, with an overrepresentation of ST-17 related strains among invasive isolates.

Conclusions:

  • A single marker is insufficient for assigning S. agalactiae isolates to specific sequence types due to high genetic recombination.
  • The developed microarray and typing scheme provide a convenient tool for genotyping large numbers of clinical and veterinary GBS isolates.
  • This genotyping approach can offer valuable insights into the epidemiology and population dynamics of Streptococcus agalactiae.