Real-time phase shift interference microscopy
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Updated: Apr 25, 2026

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
Published on: August 8, 2019
Abstract:
A real-time phase shift interference microscopy system is presented using a polarization-based Linnik interferometer operating with three synchronized, phase-masked, parallel detectors. Using this method, several important applications that require high speed and accuracy, such as dynamic focusing control, tilt measurement, submicrometer roughness measurement, and 3D profiling of fine structures, are demonstrated in 50 volumes per second and with 2 nm height repeatability.
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