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A critique of methods for temperature imaging in single cells
Guillaume Baffou1, Hervé Rigneault1, Didier Marguet2
1Institut Fresnel, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université, Centrale Marseille, Marseille, France.
Nature Methods
|August 29, 2014
Abstract:
We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting temperature heterogeneities measured in single living cells.

