[Cell cycle analysis of hybridoma cultures: study by cytofluorimetry]

An hybridoma cell line cultivated in flasks has been used as a model to study the loss of cell viability in high density cell cultures. Cell cycle analysis by cytofluorimetry has shown that a new hypochromosomic cell population appeared as soon as the viability began to decrease. However there is no evidence that this new population is constituted of dead cells.