[The RH-PA cell line--a source for obtaining the urokinase-type plasminogen activator]

A new cell line (RH-PA) was established on the basis of the human embryonic kidney cell line (RH). The new line produces urokinase type plasminogen activator (PA). The activity of the activator amounted to 150-200 IU/ml estimated with the procedure of fibrin plates. Morphological types of the RH-PA and RH cells were studied and their comparative karyologic analysis was performed. The growth curves of the cells are presented and the dynamics of PA accumulation by them in the maintaining medium is described. Optimal conditions for cultivating the RH-PA cells providing maximum production of the PA were developed.