Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: ROBIN: Reference observatory of basins for international hydrological climate change detection.

Scientific data·2025
Same author

ROBIN: Reference observatory of basins for international hydrological climate change detection.

Scientific data·2025
Same author

Type VI Collagen Deficiency Causes Enhanced Periodontal Tissue Destruction.

Journal of dental research·2024
Same author

Effect of Biomechanical Environment on Degeneration of Meckel's Cartilage.

Journal of dental research·2020
Same author

Safety and immunogenicity of the epicutaneous reactivation of pertussis toxin immunity in healthy adults: a phase I, randomized, double-blind, placebo-controlled trial.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020
Same author

Rapid and sensitive diagnostic procedure for multiple detection of pandemic Coronaviridae family members SARS-CoV-2, SARS-CoV, MERS-CoV and HCoV: a translational research and cooperation between the Phan Chau Trinh University in Vietnam and University of Bari "Aldo Moro" in Italy.

European review for medical and pharmacological sciences·2020

Related Experiment Video

Updated: Apr 25, 2026

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

3.5K

Mesenchymal stem/progenitor cell isolation from tooth extraction sockets.

R Nakajima1, M Ono1, E S Hara1

  • 1Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Journal of Dental Research
|August 30, 2014
PubMed
Summary
This summary is machine-generated.

Dental sockets offer a new source for stem cells. These dental socket-derived stem/progenitor cells (dDSCs) share characteristics with bone marrow-derived stem/progenitor cells (BMSCs) and show potential for tissue regeneration.

Keywords:
bone marrow–derived stem cellsdental socketdental tissue–derived mesenchymal stem cellsgranulation tissuestem cellswound healing

More Related Videos

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

8.8K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

25.9K

Related Experiment Videos

Last Updated: Apr 25, 2026

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

3.5K
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

8.8K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

25.9K

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Oral and Maxillofacial Surgery

Background:

  • Bone marrow-derived mesenchymal stem/progenitor cells (BMSCs) are crucial for regeneration therapy.
  • Current BMSC harvesting from the iliac crest poses risks like pain and infection.
  • Identifying alternative, accessible stem cell sources is highly desirable.

Purpose of the Study:

  • To investigate the potential of dental socket granulation tissue as a source of stem/progenitor cells.
  • To characterize and compare dental socket-derived stem/progenitor cells (dDSCs) with bone marrow-derived stem/progenitor cells (BMSCs).
  • To evaluate the regenerative capacity of dDSCs in periodontal defects.

Main Methods:

  • Granulation tissue was collected from dog dental sockets post-extraction.
  • Cells were isolated, cultured, and identified as dDSCs.
  • Phenotypic characterization using flow cytometry and differentiation assays (osteogenic, adipogenic, chondrogenic) were performed.
  • In vivo assays included ectopic bone formation and periodontal defect regeneration.

Main Results:

  • dDSCs exhibited a phenotype similar to BMSCs (CD44+, CD90+, CD271+, CD34-, CD45-).
  • dDSCs demonstrated osteogenic, adipogenic, and chondrogenic differentiation potential.
  • dDSCs showed superior colony formation, proliferation, and motility compared to BMSCs.
  • In vivo, dDSCs promoted regeneration of cementum-like, periodontal ligament-like tissues, and alveolar bone in defects.

Conclusions:

  • Dental sockets represent a novel and accessible source of stem/progenitor cells.
  • dDSCs possess characteristics and regenerative potential comparable to BMSCs.
  • These findings support the use of dDSCs in regenerative therapies, particularly for periodontal reconstruction.