Related Experiment Video
Updated: Aug 14, 2026
11:29
Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood
Published on: September 12, 2016
Cell-derived microparticles after exercise in individuals with G6PD Viangchan
Makamas Chanda1, Duangdao Nantakomol1, Daroonwan Suksom2
1Molecular Hematology Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Clinical Hemorheology and Microcirculation
|August 30, 2014
Summary
Strenuous exercise increases microparticle release in individuals with Glucose-6-phosphate dehydrogenase (G6PD) deficiency, particularly the Viangchan variant. Moderate exercise, however, does not significantly alter microparticle levels in either G6PD normal or deficient individuals.
Area of Science:
- Exercise Physiology
- Biochemistry
- Hematology
Background:
- Glucose-6-phosphate dehydrogenase (G6PD) deficiency confers sensitivity to oxidative stress.
- Microparticles (MPs) are biomarkers of cellular damage and oxidative stress.
Purpose of the Study:
- To investigate the impact of strenuous exercise (SEx) and moderate-intensity exercise (MEx) on microparticle (MP) release and antioxidant balance in G6PD-normal and G6PD-deficient individuals.
- To compare the response between G6PD normal and G6PD Viangchan variant.
Main Methods:
- Eighteen healthy females with G6PD normal and eighteen with G6PD Viangchan variant underwent SEx (maximal oxygen uptake) and MEx (75% maximal heart rate) on a treadmill.
- Measured total microparticle (TTMP) concentrations, G6PD activity, and malondialdehyde (MDA) levels.
- Utilized cell-specific antibodies to identify MP origins.
Main Results:
- SEx significantly increased TTMPs in G6PD normal individuals, with a more pronounced elevation in G6PD Viangchan variant.
- MEx did not alter TTMP levels in either group.
- TTMP concentrations showed an inverse correlation with G6PD activity and a positive correlation with MDA levels.
- MPs were primarily derived from platelets and erythrocytes.
Conclusions:
- G6PD Viangchan variant individuals experience higher microparticle release following strenuous exercise compared to G6PD normal individuals.
- Moderate-intensity exercise appears safe regarding microparticle generation and oxidative stress in both G6PD normal and Viangchan variant individuals.
- G6PD activity is a key factor in modulating exercise-induced oxidative stress and microparticle formation.
