Related Experiment Video
Updated: Sep 18, 2026

12:50
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
Published on: August 9, 2011
Direct isolation (without enrichment) of Listeria monocytogenes in milk
L Dominguez1, V Briones, M Blanco
1Department of Animal Pathology, Microbiology and Immunity, Veterinary Faculty, Madrid, Spain.
Abstract:
In the present work we have developed a direct method (without enrichment) for the isolation of listeriae in milk, using the combinated action of SDS (sodium dodecyl sulphate), centrifugation and selective media. It is possible to identify even 0.5 listeriae/ml amongst 7.2 x 10(7) c.f.u. contaminants/ml.

