Related Experiment Video
Updated: Apr 25, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
Detection of the interaction between host and bacterial proteins: eukaryotic nucleolin interacts with Francisella
1INSERM 1151 Team 11, Unité de Pathogénie des Infections Systémiques, Université Paris Descartes, Bâtiment Leriche, 14 rue Maria Helena Vieira Da Silva CS61431, 75993, Paris cedex 14, France, monique.barel@inserm.fr.
Abstract:
Dissecting the interaction between bacterial and host proteins is fundamental in understanding pathogenesis. It is also very helpful for exploring new therapeutic approaches, either preventive or curative. Here, we describe different techniques, which allowed us to detect new molecules involved in the binding and infection of the bacterium Francisella tularensis, on human cells. This facultative intracellular pathogen is the causative agent of tularemia and is considered as a bio-threatening agent. The privileged host cells are monocytes and macrophages. We used both "in vitro" and "in vivo" experiments to explore the modulation of F. tularensis infection and thereafter determine a bacterial ligand and its host receptor molecule.
Related Concept Videos
Transcription Elongation Factors
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
Transcription Elongation Factors
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Coordination of Gene Expression Processes in Bacteria
Intracellular Movement of Viruses and Bacteria
Regulation of Nuclear Protein Sorting

