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Updated: Jan 29, 2026

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
Published on: June 10, 2016
Human neural crest stem cells derived from human pluripotent stem cells
Qiuyue Liu1, Andrzej Swistowski, Xianmin Zeng
1Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA, 94945, USA.
Abstract:
The neural crest cells give rise to neurons and glia in the peripheral nervous system, which is an important component of the nervous system. Here we developed a scalable process of inducing neural crest stem cells (NCSCs) from hESCs/iPSCs by a combination of growth factors in medium conditioned on stromal cells, and showed that NCSCs could be purified by p75 using FACS. In vitro-expanded NCSCs were able to differentiate into neurons and glia (Schwann cells) of the peripheral nervous system (PNS) as well as mesenchymal derivatives.
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