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Adipose-derived stem cells: methods for isolation and applications for clinical use.
Brian Mailey1, Ava Hosseini, Jennifer Baker
1Department of Surgery, University of California San Diego, San Diego, CA, USA, scohen@facesplus.com.
Methods in Molecular Biology (Clifton, N.J.)
|September 1, 2014
Summary
Researchers are exploring regenerative cells from fat, known as adipose-derived stem cells (ADSC), for various therapies. Laboratory methods for isolating ADSC and their clinical applications are reviewed.
Area of Science:
- Adipose tissue sciences
- Regenerative medicine
- Stem cell biology
Background:
- The stromal vascular fraction (SVF) of adipose tissue contains regenerative cells.
- Adipose-derived stem cells (ADSC) are a key component of the SVF.
- SVF isolation is achievable through laboratory or operating room procedures.
Purpose of the Study:
- To review laboratory methods for isolating ADSC.
- To summarize ongoing clinical trials utilizing ADSC for cell therapy.
- To highlight the broad therapeutic potential of ADSC across medical specialties.
Main Methods:
- Enzymatic digestion of adipose tissue.
- Concentration of the stromal vascular fraction (SVF).
- Isolation and potential expansion of adipose-derived stem cells (ADSC).
Main Results:
- Efficient isolation of SVF containing ADSC is possible.
- ADSC can be used directly, with scaffolds, or after expansion.
- Numerous clinical trials are investigating ADSC efficacy.
Conclusions:
- ADSC hold significant promise for cell-based therapeutics.
- Ongoing research spans cardiology, neurology, immunology, and surgery.
- Further investigation into ADSC is crucial for advancing regenerative medicine.
