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Updated: Apr 25, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
Fluorescence imaging of viscous materials in the ultraviolet-visible wavelength range
Patrik J Murr1, Markus S Rauscher1, Anton Tremmel1
1Institute for Measurement Systems and Sensor Technology, Technische Universität München, Theresienstraße 90, 80333 München, Germany.
Abstract:
This paper presents an approach of an innovative measurement principle for the quality control of viscous materials during a manufacturing process based on fluorescence imaging. The main contribution to the state of the art provided by this measurement system is that three equal fluorescence images of a static or moving viscous object are available in different optical paths. The independent images are obtained by two beam splitters which are connected in series. Based on these images, it is possible to evaluate each image separately. In our case, three optical bandpass filters with different center wavelengths of 405 nm, 420 nm, and 440 nm were used to filter the separate fluorescence images. The developed system is useable for the detection of impurities in the micrometer range. Further, incorrect mixing ratios of particular components and wrong single components in the viscous materials can be detected with the setup. Moreover, it is possible to realize static and dynamic measurements. In this case the maximum speed of the objects was 0.2 m/s for the dynamic measurements. Advantages of this measurement setup are the universality due to the use of optical standard components, the small dimension and the opportunity to integrate it easily into ongoing processes. In addition, the measurement system works on a non-contact basis. Thus, the expense for maintenance is at a very low level compared to currently available measurement setups for the investigated application. Furthermore, the setup provides for the first time a simultaneous analysis of more than one component and the detection of impurities concerning their nature and size in a manufacturing process.
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