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Related Experiment Video

Updated: Apr 25, 2026

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

Fluorescent sensors reveal subcellular thermal changes.

Reiko Sakaguchi¹, Shigeki Kiyonaka², Yasuo Mori³

¹World Premier International Research Initiative-Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8501, Japan.

Current Opinion in Biotechnology

|September 1, 2014

Summary

Studying cellular heat production is crucial for understanding thermoregulation. Genetically encoded thermosensors allow visualization of thermal changes within cells, aiding physiological discoveries.

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Last Updated: Apr 25, 2026

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High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

Published on: April 16, 2017

Area of Science:

Cellular Biology
Physiology
Biophysics

Background:

Thermoregulation is vital for mammals and birds.
Thermogeneration mechanisms are localized in subcellular organelles.
Studying organelle thermogenesis in intact cells is challenging.

Purpose of the Study:

To review and compare intracellular thermosensors.
To focus on genetically encoded sensors.
To explore physiological discoveries using thermal imaging.

Main Methods:

Review of intracellular thermosensor literature.
Focus on genetically encoded fluorescent sensors.
Analysis of imaging techniques for thermal changes.

Main Results:

Identification of diverse intracellular thermosensors.
Highlighting genetically encoded sensors for cellular thermogenesis studies.
Demonstrating the utility of thermal imaging in physiological research.

Conclusions:

Intracellular thermosensors, especially genetically encoded ones, are essential tools.
Thermal imaging provides critical spatio-temporal data on cellular thermogenesis.
These methods are indispensable for advancing thermal biology research.