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Updated: Apr 25, 2026
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
Published on: January 30, 2018
The inter-individual variability outperforms the intra-individual variability of differentially expressed proteins
A Gürtler1, M Hauptmann, S Pautz
1Federal Office for Radiation Protection, Department SG Radiation Protection and Health , Neuherberg , Germany .
Context:
Radio-sensitivity in normal tissue is characterized by heterogeneity throughout the population and the absence of pre-diagnostic biomarkers.
Objective:
We conducted a proteomic approach to search for radiation characteristic protein regulation.
Materials And Methods:
Cell lines were 10 Gy irradiated and analysed by 2D-DIGE after 24 h. RESULTS were analysed intra- and inter-individually. The principal component analysis and hierarchical clustering was applied to all datasets.
Results:
Differences in intra-individual spot abundance prior and post irradiation exactly show the separation of sample classes in two groups: sham-irradiated and irradiated. The inter-individual datasets clustered according to the cell line. The intra-individual differences on protein level after gamma-irradiation are very low, compared with the inter-individual differences among cell lines derived from the same tissue.
Conclusion:
The application of 2-D DIGE may offer a realistic chance for a better molecular characterization of radio-sensitivity and for the discovery of candidate biomarkers.
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