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Updated: Apr 25, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
Technologies and strategies to characterize and quantitate metabolites in drug discovery and development
Selvan Ravindran1, Amol Jadhav, Prashant Surve
1Departments of Bio-Analytical and Biotransformation, Drug Metabolism and Pharmacokinetics, Sai Life Sciences Limited, Building 1, Plot No 2, Chrysalis Enclave, International Bio-Tech Park, Phase-II, Hinjewadi, Pune-, 411 057, Maharashtra, India.
Abstract:
Pharmacokinetics and toxicokinetics studies rely on the quantitation of drugs and its metabolites. Drug metabolism studies are based on the characterization of the structure of drugs and their metabolites. Liquid chromatography coupled with mass spectrometry is an undisputed technology for the routine analysis of drugs and metabolites in the drug discovery set-up. Advancements in liquid chromatography and mass spectrometry have accelerated and improved drug discovery and development. Identification and quantitation of drugs and metabolites have gained importance because of their relevance to clinical research. In the present review, we present a bird's eye view of qualitative and quantitative analysis of drugs and their metabolites. Various technologies available to characterize and quantitate metabolites are analyzed. Characterization of metabolites of the sulfonyl urea drug glyburide and the kinetics of glyburide metabolism are discussed in detail.
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