Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Myelin basic protein is an RNA chaperone in microglial nuclear retro-transport.

bioRxiv : the preprint server for biology·2026

Same author

Spatially tunable multiomic sequencing using light-driven combinatorial barcoding of molecules in tissues.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Stem cell treatments and Parkinson's disease: Science and misconceptions.

Journal of Parkinson's disease·2026

Same author

Degradation of G-quadruplex-binding proteins in chromatin using G4-ligand-based proteolysis-targeting chimeras.

Nature chemistry·2026

Same author

Dissecting the Binding Interactions of the Chromatin Remodeler SMARCA4 with G-Quadruplex DNA.

Biochemistry·2026

Same author

Sequencing DNA methylation and hydroxymethylation at co-occurring chromatin features.

Nature communications·2026

Search research articles

Related Experiment Video

Updated: Apr 25, 2026

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

oxBS-450K: a method for analysing hydroxymethylation using 450K BeadChips.

Sabrina K Stewart¹, Tiffany J Morris¹, Paul Guilhamon¹

¹Department of Cancer Biology, UCL Cancer Institute, University College London, London WC1E 6BT, UK.

Methods (San Diego, Calif.)

|September 2, 2014

Summary

This summary is machine-generated.

This study introduces oxBS-450K, a new method for DNA methylation analysis. It accurately distinguishes 5-methylcytosine and 5-hydroxymethylcytosine on 450K BeadChips for biomedical research.

Keywords:

450K BeadChip Bisulfite conversion DNA methylation Epigenetics Hydroxymethylation Oxidation

More Related Videos

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Published on: September 26, 2015

DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Related Experiment Videos

Last Updated: Apr 25, 2026

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Published on: September 26, 2015

DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Area of Science:

Epigenetics
Molecular Biology
Genomics

Background:

DNA methylation analysis is crucial in biomedical research.
The 450K BeadChip is widely used for epigenome-wide association studies.
Traditional bisulfite methods cannot differentiate 5-methylcytosine (5mC) from 5-hydroxymethylcytosine (5hmC).

Purpose of the Study:

To adapt oxidative bisulfite (oxBS) chemistry for 450K BeadChips.
To enable simultaneous detection of 5mC and 5hmC in a single workflow.
To introduce the oxBS-450K protocol for enhanced DNA methylation analysis.

Main Methods:

Adaptation of oxidative bisulfite (oxBS) chemistry.
Application to Infinium HumanMethylation450 (450K) BeadChip platform.
Validation using mass spectrometry and pyrosequencing.

Main Results:

Successful adaptation of oxBS chemistry to 450K BeadChips, creating the oxBS-450K protocol.
Demonstrated reproducible (R(2)>0.99) detection of 5hmC.
Validated the protocol's accuracy in human brain tissue samples.

Conclusions:

The oxBS-450K protocol allows specific detection of both 5mC and 5hmC.
This method enhances DNA methylation analysis capabilities for high-throughput studies.
The optimized protocol offers a reliable tool for epigenomic research.