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Updated: Aug 4, 2026

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Published on: November 2, 2018
Does NF-kappa B relieve the transcription block in c-myc?
1Division of Biophysical Sciences, National Accelerator Centre, Faure, South Africa.
Abstract:
In this report, it is shown that the mRNA of the c-myc oncogene is capable of forming an extensive stem-and-loop structure, with a free energy of delta G (25 degrees C) = -34 kcal. This secondary structure is situated at the 3' end of the first exon, immediately upstream of an elongation block. It is shown that this region contains potential binding sites for 3 different activator proteins, namely AP-1, AP-2, and nuclear factor-kappa B (NF-kappa B). From an analysis of the properties of these proteins, NF-kappa B could be identified as a candidate for the trans-acting factor involved in relieving the block to transcription.
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