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Bioelectrical conversion in sensors with living cells.

H Vais1, I Ardelean, D G Mărgineanu

  • 1Institute of Biological Sciences, Bucharest, Romania.

Physiologie (Bucarest)
|October 1, 1989
PubMed
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This study developed bacterial electrodes using Pseudomonas sp. for glucose detection in body fluids and biofuel cells with Clostridium sp. for monitoring bacterial cultures in fermentative reactors.

Area of Science:

  • Biotechnology
  • Microbial electrochemistry
  • Biosensors

Background:

  • Bacterial metabolism is crucial for biotechnological applications like biosensors and biofuel cells.
  • Developing efficient biosensors and monitoring tools for bacterial cultures is essential for industrial processes.

Purpose of the Study:

  • To construct a bacterial electrode with Pseudomonas sp. for glucose detection in biological fluids.
  • To investigate biofuel cells utilizing anaerobic bacteria (Clostridium sp.) for monitoring bacterial culture states.

Main Methods:

  • Construction of a bioselective bacterial electrode using Pseudomonas sp.
  • Development of biofuel cells employing anaerobic Clostridium sp.
  • Amperometric measurement of glucose concentration and bacterial culture status.

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Main Results:

  • The bacterial electrode demonstrated linear glucose detection in the 2.5 x 10(-5)-1.25 x 10(-4) M range with a sensitivity limit of 10(-5) M.
  • The electrode exhibited a time constant of 10 minutes and operational stability exceeding ten days.
  • Biofuel cells provided an amperometric response reflecting the state of Clostridium sp. cultures, suitable for automated control.

Conclusions:

  • Bacterial electrodes offer a viable method for probing glucose in body fluids.
  • Clostridium sp.-based biofuel cells serve as a promising tool for real-time monitoring and automated control of bacterial suspensions in fermentation.
  • This research highlights the biotechnological potential of bacterial metabolism in biosensing and process control.