Generation and characterization of three monoclonal antibodies to human ovarian epithelial adenocarcinomas

Three murine monoclonal antibodies COCL66-9, COC183A5 and COC183B2 were raised against human epithelial ovarian adenocarcinoma with soluble antigens. The monoclonal antibodies were stable after culture and stored under 80 C for 9 to 20 months. The subclass and titre were all examined and the reactivities were tested on various paraffin embedded tissue sectionism by immunoperoxidase stainings. The results of positive staining against ovarian epithelial adenocarcinoma were 77.7%, 87.2% and 75.6% for COC166-9, COC183A5 and COCL83B2 respectively. Human ovarian carcinoma xenograft in nude mice were radioimaged after 131I-COC183B2 administration. Gamma scintigraphy demonstrated specific localization of the radiolabeled monoclonal antibody on these tumors. The results showed a bright future for immunodiagnosis and immunotherapy.