Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

6.3K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
6.3K
Fibril-associated Collagen01:11

Fibril-associated Collagen

2.7K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
2.7K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prescription writing, psychotropic drug use, and medication adherence in a tertiary care hospital in southern India: a prospective observational study.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2025
Same author

Signal recovery from stimulation artifacts in intracranial recordings with dictionary learning.

Journal of neural engineering·2020
Same author

Anomalous Antiferromagnetism in Metallic RuO_{2} Determined by Resonant X-ray Scattering.

Physical review letters·2019
Same author

Tissue engineering and regenerative approaches to improving the healing of large bone defects.

European cells & materials·2016
Same author

Differential bleaching of corals based on El Niño type and intensity in the Andaman Sea, southeast Bay of Bengal.

Environmental monitoring and assessment·2016
Same author

Cell therapy for bone repair: narrowing the gap between vision and practice.

European cells & materials·2014
Same journal

Stable, micron-scale lipocondensates achieving prolonged circulation without PEGlyated lipids.

Biomaterials science·2026
Same journal

Fiber and continuum scale contributions to the intrinsic and apparent fracture of soft collagenous tissue <i>via</i> cutting.

Biomaterials science·2026
Same journal

Surface morphology-regulated tissue adhesion in solid and mesoporous silica-reinforced gelatin nanocomposite hydrogels.

Biomaterials science·2026
Same journal

Nanostructured hyaluronic acid-chia mucilage film as bioactive wound dressings for accelerated skin regeneration.

Biomaterials science·2026
Same journal

Tunable bio-inspired hybrid hydrogels reprogram stem cell-derived extracellular vesicles for superior wound regeneration.

Biomaterials science·2026
Same journal

Bioorthogonally reinforced injectable granular hydrogels synergizing ECM mimicry with microporosity for skin tissue engineering.

Biomaterials science·2026
See all related articles

Related Experiment Video

Updated: Apr 24, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

12.2K

Vasculogenesis and Angiogenesis in Modular Collagen-Fibrin Microtissues.

A W Peterson1, D J Caldwell1, A Y Rioja1

  • 1Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.

Biomaterials Science
|September 2, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed collagen-fibrin microbeads to engineer vascular networks. These engineered blood vessel structures show potential for tissue regeneration and treating ischemic conditions.

More Related Videos

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

20.7K
Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
12:13

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization

Published on: October 28, 2013

10.0K

Related Experiment Videos

Last Updated: Apr 24, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

12.2K
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

20.7K
Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
12:13

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization

Published on: October 28, 2013

10.0K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • New blood vessel formation is essential for tissue development, repair, and regeneration.
  • Modular tissue engineering offers a bottom-up strategy for constructing complex tissues, including vascular networks.

Purpose of the Study:

  • To fabricate and characterize collagen-fibrin composite microbeads for vascular network formation.
  • To investigate the potential of these microbeads as a modular system for studying vasculogenesis and angiogenesis.

Main Methods:

  • Collagen-fibrin composite microbeads (100-300 μm) were created using a water-in-oil emulsion technique.
  • Human endothelial cells and fibroblasts were embedded within the microbeads.
  • Microbeads were cultured as free-floating populations or within a fibrin gel for 14 days.

Main Results:

  • The collagen-fibrin matrix supported cell viability, spreading, and the formation of endothelial cell networks within 7-14 days.
  • Fibroblasts exhibited pericyte-like behavior, and laminin deposition indicated vessel maturation.
  • Embedded microbeads promoted vasculogenesis and angiogenesis, with observed inosculation and lumen formation.

Conclusions:

  • Collagen-fibrin composite microbeads effectively support the formation of vascular networks.
  • This modular microtissue system can be used to study new vessel formation processes.
  • The system holds promise for therapeutic applications in treating ischemic conditions.