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Chemical biology of genomic DNA: minimizing PCR bias
Gordon R McInroy1, Eun-Ang Raiber, Shankar Balasubramanian
1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. sb10031@cam.ac.uk.
Abstract:
The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.
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