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Updated: Apr 24, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

OncomiR detection in circulating body fluids: a PDMS microdevice perspective.

Cristina Potrich¹, Valentina Vaghi, Lorenzo Lunelli

¹Fondazione Bruno Kessler-LaBSSAH, Via Sommarive 18, Trento, Italy. cpotrich@fbk.eu.

|September 2, 2014

Summary

This summary is machine-generated.

Researchers developed a novel PDMS microdevice for rapid purification and reverse transcription of circulating microRNAs (miRNAs). This innovation simplifies biomarker assays for early cancer detection.

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Last Updated: Apr 24, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

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Published on: June 8, 2019

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

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Area of Science:

Biomedical Engineering
Molecular Diagnostics
Oncology Biomarkers

Background:

Circulating microRNAs (miRNAs) show promise as minimally invasive diagnostic biomarkers in oncology.
Lab-on-a-chip devices are under development for efficient purification and detection of miRNAs from biological fluids.

Purpose of the Study:

To develop an innovative polydimethylsiloxane (PDMS)-based microdevice for the purification and reverse transcription of circulating miRNAs.
To optimize PDMS surface functionalization for enhanced miRNA capture and streamline diagnostic workflows.

Main Methods:

Fabrication and functionalization of a PDMS parallel device with 3-aminopropyltriethoxysilane (APTES) and poly(ethylene glycol) silanes (PEG-s).
Characterization of functionalized surfaces and assessment of synthetic and cell-free miRNAs adsorption.
On-chip purification and reverse transcription of miRNAs, followed by quantification using RT-qPCR and ddPCR.

Main Results:

Identified an optimal PDMS functionalization (0.1% APTES/0.9% PEG-silane) for efficient capture of both synthetic and extracellular miRNAs.
Demonstrated direct on-chip reverse transcription of purified miRNA, significantly reducing assay time.
Confirmed high purification efficiency and simplified workflow for circulating miRNA isolation from biological samples.

Conclusions:

The developed PDMS microdevice offers a rapid, user-friendly solution for circulating miRNA purification and reverse transcription.
This technology advances the development of diagnostic and prognostic assays for oncomiR-based cancer detection.