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Updated: Apr 24, 2026

Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

Isolation of murine lymph node stromal cells.

Maria A S Broggi¹, Mathias Schmaler¹, Nadège Lagarde¹

¹Department of Biomedicine, Immunoregulation, University of Basel and University Hospital Basel.

Journal of Visualized Experiments : Jove

|September 2, 2014

Summary

This study introduces a new method for isolating lymph node stromal cells. The protocol uses short enzymatic digestion and mechanical disruption to preserve cell viability and surface marker expression for better research.

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Last Updated: Apr 24, 2026

Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification

Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification

Published on: November 1, 2007

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Published on: February 28, 2018

Area of Science:

Immunology
Cell Biology
Anatomy

Background:

Secondary lymphoid organs, like lymph nodes, contain stromal cells crucial for lymphocyte homeostasis and function.
Stromal cell subsets, identified by markers like CD31 and podoplanin (gp38), perform diverse roles including structural support and immune cell interaction.
Emerging evidence highlights stromal cells' role in regulating cytotoxic T cell responses.

Purpose of the Study:

To develop an improved protocol for isolating viable lymph node stromal cells.
To maintain surface molecule expression on stromal cells during isolation for accurate downstream analysis.
To overcome limitations of current enzymatic digestion methods that cause cell death and marker loss.

Main Methods:

A novel protocol combining short enzymatic digestion with automated mechanical disruption was employed.
The method focuses on obtaining a single-cell suspension of lymph node stromal cells.
The protocol aims to minimize damage to stromal cells and preserve their surface markers.

Main Results:

The proposed method yields viable single-cell suspensions of lymph node stromal cells.
Surface molecule expression on isolated stromal cells is maintained, unlike traditional methods.
This technique offers a more reliable approach for studying stromal cell populations and functions.

Conclusions:

A refined protocol for lymph node stromal cell isolation has been established.
This method enhances cell viability and preserves crucial surface markers.
The improved isolation technique facilitates more accurate research into stromal cell biology and immune regulation.