Developmental validation of the PowerPlex(®) ESI 16/17 Fast and PowerPlex(®) ESX 16/17 Fast Systems
Robert S McLaren1, Jeanne Bourdeau-Heller1, Jaynish Patel1
1Promega Corporation, Madison, WI 53711-5399, USA.
Forensic Science International. Genetics
|September 3, 2014
Summary
New PowerPlex® ESI and ESX Fast Systems offer rapid DNA amplification from various sample types, including direct amplification from swabs and FTA cards. These validated systems ensure concordance and sensitivity for forensic DNA analysis.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Background:
- The PowerPlex® ESI and ESX Systems were developed in 2009 to meet European forensic group requirements for STR multiplexes.
- Faster cycling versions were developed to improve efficiency in DNA analysis.
Purpose of the Study:
- To perform developmental validation of the new PowerPlex® ESI Fast and ESX Fast Systems.
- To assess their performance for direct amplification and compatibility with various sample types and platforms.
Main Methods:
- Developmental validation following SWGDAM and DAB guidelines.
- Testing direct amplification from blood and buccal samples on FTA and non-FTA paper, and SwabSolution™ extracts.
- Assessing concordance with original PowerPlex® ESI and ESX Systems.
Main Results:
- No changes in autosomal primer pair sequences, showing full concordance with original systems.
- Sensitive detection of low DNA levels and robust profiles from high input DNA.
- Tolerance to PCR inhibitors and variations in reaction components.
- Compatibility with multiple thermal cyclers and capillary electrophoresis platforms.
Conclusions:
- The PowerPlex® ESI Fast and ESX Fast Systems are validated for forensic DNA analysis.
- These systems offer rapid, sensitive, and robust DNA profiling from diverse sample types.
- Their compatibility with various equipment enhances their utility in forensic laboratories.
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