Related Experiment Video
Updated: Apr 24, 2026
12:47
Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
9.0K
Correction of MS data for naturally occurring isotopes in isotope labelling experiments
Pierre Millard1, Fabien Letisse, Serguei Sokol
1Université de Toulouse; INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, Toulouse, 31077, France.
Methods in Molecular Biology (Clifton, N.J.)
|September 3, 2014
Summary
IsoCor software corrects raw mass spectrometry data for natural isotopes, enabling accurate quantitative analysis in biological and metabolic studies. This tool enhances the precision of isotope tracing experiments for various applications.
Area of Science:
- Metabolomics
- Biochemistry
- Analytical Chemistry
Background:
- Mass spectrometry (MS) coupled with isotope labeling is crucial for studying metabolism and biological processes.
- Quantitative analyses, such as metabolic flux analysis, necessitate correcting raw MS data for natural isotope contributions.
Purpose of the Study:
- To present IsoCor, a user-friendly software for correcting raw mass spectrometry data for natural isotopes.
- To enable accurate quantitative isotopic studies using various tracers and improve data analysis for modern MS methods.
Main Methods:
- Description of the IsoCor software for correcting isotopic clusters in mass spectrometry data.
- Application of IsoCor for correcting contributions from naturally abundant isotopes.
- Utilizing IsoCor for various isotopic tracers, including common (e.g., 13C, 15N, 18O) and unusual (e.g., 57Fe, 77Se) isotopes.
- Incorporating options for enhanced accuracy, such as correcting for tracer isotopic purity.
Main Results:
- IsoCor provides automated correction for large datasets generated by modern MS techniques.
- The software facilitates accurate quantitative analysis by correcting for natural isotope abundance.
- IsoCor supports a wide range of isotopic tracers, enhancing the flexibility of quantitative studies.
Conclusions:
- IsoCor is a flexible and user-friendly tool for accurate quantitative mass spectrometry analysis.
- The software improves the reliability of isotope tracing experiments in biological research.
- Automated correction capabilities of IsoCor support high-throughput data analysis in metabolomics.
Related Concept Videos
01:13Mass Spectrometry: Isotope Effect
4.3K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
4.3K
01:19Mass Spectrometry: Overview
8.1K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
8.1K
01:24Mass Spectrum: Interpretation
4.1K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
4.1K
01:15High-Resolution Mass Spectrometry (HRMS)
2.6K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.6K
01:16Mass Spectrometers
9.6K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
9.6K
01:14NMR Spectrometers: Resolution and Error Correction
989
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
989