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Methyl-thiophanate increases reactive oxygen species production and induces genotoxicity in rat peripheral blood

Ibtissem Ben Amara1, Hajer Ben Saad, Boutheina Cherif

  • 1Animal Physiology Laboratory, Faculty of Sciences, University of Sfax , Tunisia .

Toxicology Mechanisms and Methods
|September 3, 2014
PubMed

Insights

Methylthiophanate fungicide causes short-term hematotoxicity and genotoxicity in rats, indicated by blood cell damage and oxidative stress. These adverse effects, including increased oxidative stress markers, were observed 24 hours post-injection but showed improvement after 72 hours.

Area of Science:

  • Toxicology
  • Environmental Science
  • Biochemistry

Background:

  • Methylthiophanate is a widely used fungicide for crop disease control.
  • Understanding its toxicological profile is crucial for assessing environmental and health risks.

Purpose of the Study:

  • To investigate the short-term hematotoxicity and genotoxicity of methylthiophanate in adult rats.
  • To elucidate the role of oxidative stress in methylthiophanate-induced toxicity.

Main Methods:

  • Adult rats were administered intraperitoneal injections of methylthiophanate at doses of 300, 500, and 700 mg/kg body weight.
  • Hematological parameters, erythrocyte osmotic fragility, oxidative stress markers (hydrogen peroxide, advanced oxidation of protein products, malondialdehyde), and antioxidant enzyme activities (superoxide dismutase, glutathione peroxidase) were assessed at 24, 48, and 72 hours post-injection.
  • Genotoxicity was evaluated by assessing micronuclei frequency and cell viability in white blood cells (WBC).

Main Results:

  • Methylthiophanate administration led to significant hematological perturbations, including decreased red blood cells and hemoglobin content, and increased erythrocyte osmotic fragility at 24 and 48 hours.
  • Genotoxic effects were observed in WBC, characterized by increased micronuclei frequency and decreased cell viability.
  • Elevated levels of oxidative stress markers and increased antioxidant enzyme activities were noted 24 hours post-treatment, suggesting the involvement of oxidative stress.
  • All assessed parameters showed improvement by 72 hours post-injection.

Conclusions:

  • Short-term exposure to methylthiophanate induces hematotoxicity and genotoxicity in rats.
  • Oxidative stress plays a significant role in the toxic effects of methylthiophanate.
  • The fungicide exhibits pro-oxidant and genotoxic properties, impacting the defense system, though recovery is observed within 72 hours.
Keywords:
Genotoxicityhematological parametersmethylthiophanatemicronucleus testoxidative stressrat viability test

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