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Published on: May 2, 2018
Static magnetic field attenuates lipopolysaccharide-induced multiple organ failure: a histopathologic study in mice
Wei-Yi Lai1, Yu-Chih Huang, Wei-Jen Chang
1School of Dentistry, College of Oral Medicine, Taipei Medical University , Taipei , Taiwan.
Purpose:
Previous studies demonstrated that static magnetic fields (SMF) were effective in down-regulating the expression of lipopolysaccharide (LPS)-induced inflammatory cytokines. The aim of this study was to provide histological evidence of SMF attenuating LPS-induced multiple organ failure (MOF).
Materials And Methods:
In this study, BALB/cByJNarl (5 weeks, weighing 20-25 g) mice were chosen as test subjects. The tested animals were challenged with 50 mg/kg LPS after they were exposed to a continuous SMF for 2 h. The survival rate and pathological changes in lungs, kidneys, and livers of the LPS- challenged mice were examined with and without SMF treatment. In addition, the effects of SMF exposure on body temperature control of the LPS-challenged mice were monitored.
Results:
Our results showed that at 30 h the survival rate of LPS-challenged mice increased 3.6-fold (p < 0.05). In addition, 6 h after LPS injection, the average body temperature of SMF-exposed mice was 1.07°C lower than that of unexposed animals. Tissue biopsies demonstrated that SMF exposure reduced damage to the lungs, livers, and kidneys in the LPS-challenged mice.
Conclusions:
SMF show potential as a viable prophylactic alternative for controlling LPS-induced MOF.
Insights
Static magnetic fields (SMF) significantly improved survival rates in mice challenged with lipopolysaccharide (LPS)-induced multiple organ failure (MOF). SMF also reduced organ damage, offering a potential prophylactic treatment for MOF.
Area of Science:
- Biomedical Engineering
- Immunology
- Pathology
Background:
- Static magnetic fields (SMF) have shown efficacy in reducing inflammatory cytokine expression.
- Lipopolysaccharide (LPS) is a potent inducer of inflammation and multiple organ failure (MOF).
Purpose of the Study:
- To provide histological evidence that SMF attenuates LPS-induced MOF.
- To investigate the protective effects of SMF on organ damage in a mouse model.
Main Methods:
- BALB/cByJNarl mice were exposed to SMF for 2 hours before LPS challenge.
- Survival rates, pathological changes in lungs, kidneys, and livers, and body temperature were monitored.
- Histological examination assessed organ damage in SMF-treated and untreated groups.
Main Results:
- SMF exposure increased the survival rate of LPS-challenged mice by 3.6-fold.
- SMF treatment led to a significant reduction in lung, liver, and kidney damage.
- Body temperature in SMF-exposed mice was lower post-LPS challenge.
Conclusions:
- SMF demonstrates potential as a prophylactic intervention against LPS-induced MOF.
- Histological evidence supports the protective role of SMF in mitigating organ damage.
