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Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

Notch Signaling Pathway

Notch Signaling Pathway

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Integrins

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
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Activation of Integrins

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Related Experiment Video

Updated: Apr 24, 2026

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

Integrins regulate epithelial cell differentiation by modulating Notch activity.

M Jesús Gómez-Lamarca¹, Laura Cobreros-Reguera¹, Beatriz Ibáñez-Jiménez¹

¹Centro Andaluz de Biología del Desarrollo CSIC-University Pablo de Olavide, Sevilla 41013, Spain.

Journal of Cell Science

|September 3, 2014

Summary

Integrin signaling controls cell cycle exit and differentiation during development. This pathway regulates the switch from mitosis to endocycle and impacts Notch signaling in Drosophila follicle cells.

Keywords:

Differentiation Integrins Proliferation

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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

Area of Science:

Developmental Biology
Cell Signaling
Epithelial Biology

Background:

Coordinating cell cycle exit with differentiation is vital for development and tissue homeostasis.
Dysregulation of this process can lead to organogenesis defects and cancer.
Known regulators include Notch, Salvador-Warts-Hippo (SWH) pathways, and myosin activity.

Purpose of the Study:

To identify novel developmental signals regulating the switch from cell cycle exit to differentiation.
To investigate the role of integrin signaling in this developmental switch.
To elucidate the mechanisms by which integrins influence cell cycle and differentiation.

Main Methods:

Utilized Drosophila ovary follicle cell development as a model system.
Generated and analyzed integrin loss-of-function mutants.
Assessed cell cycle status, differentiation markers, and signaling pathway activity (Notch, SWH).

Main Results:

Integrin signaling disruption blocked the mitosis-to-endocycle switch and differentiation in posterior follicle cells (PFCs).
Integrin function regulates the cyclin-dependent kinase inhibitor (CKI) dacapo.
Integrin mutants exhibited impaired Notch signaling and endocytosis, which were rescued by modulating Notch pathway components.

Conclusions:

Integrin signaling is a novel, critical regulator of epithelial cell differentiation during development.
Integrins directly modulate the Notch pathway's activity to control cell cycle exit and differentiation timing.
Findings reveal integrin's role in the spatial and temporal regulation of developmental processes.