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An improved method for rapid generation and screening of Bacillus thuringiensis phage-resistant mutants
Annika Gillis1, Jacques Mahillon1
1Laboratory of Food and Environmental Microbiology, Université catholique de Louvain, Croix du Sud, Louvain-la-Neuve, Belgium.
Journal of Microbiological Methods
|September 3, 2014
Abstract:
A simple method to isolate, screen and select phage-resistant mutants of Bacillus thuringiensis was developed. The traditional double-layer agar method was improved by a combination of the spotting assay using a lytic phage, to generate the bacterial-resistant mutants, with an inverted spotting assay (ISA), to rapidly screen the candidate-resistant mutants.

