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Related Experiment Video

Updated: Apr 24, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Toward larger DNA origami.

Alexandria N Marchi¹, Ishtiaq Saaem, Briana N Vogen

¹Biomedical Engineering Department, Duke University , Durham, North Carolina 27708, United States.

|September 3, 2014

Summary

This summary is machine-generated.

Researchers developed a larger DNA scaffold and cost-effective synthesis for DNA origami. This enables the creation of larger, more complex nanostructures, advancing DNA nanotechnology and materials science.

Keywords:

DNA origami Nanotechnology hybrid bacteriophage lambda DNA on-chip DNA synthesis structural DNA nanotechnology

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Published on: March 5, 2019

Area of Science:

Structural DNA nanotechnology
Biomolecular engineering
Materials science

Background:

DNA origami is a powerful bottom-up fabrication method for nanostructures.
Current scaffold lengths limit the scale and increase costs of DNA origami.
Larger scaffolds are needed for advanced nanodevice fabrication.

Purpose of the Study:

To overcome limitations in DNA origami scaffold size and synthesis cost.
To enable the production of larger, more complex DNA nanostructures.
To demonstrate efficient assembly of large-scale DNA origami.

Main Methods:

Production of a 51,466-nucleotide circular single-stranded DNA scaffold using a λ/M13 hybrid virus.
Inexpensive DNA synthesis of staple strands using inkjet printing on functionalized micropillars.
Experimental assembly of a 51-kilobasepair DNA origami structure.

Main Results:

Demonstrated efficient assembly of a large-scale (51 kbp) DNA origami using the novel scaffold.
Created 2D asymmetric origami sheets with controlled global curvature.
Verified predictable substrate landing orientations using atomic force microscopy.

Conclusions:

The developed methods enable the production of the largest biologically derived DNA scaffolds to date.
Cost-effective staple strand synthesis significantly reduces the financial barrier for large DNA origami.
This advancement facilitates the creation of larger and more complex nanometer-scale materials and devices.