Related Experiment Video
Updated: Apr 24, 2026
08:33
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
Published on: November 17, 2018
15.5K
Oral microbiome composition changes in mouse models of colitis.
Jaana Rautava1, Lee J Pinnell, Linda Vong
1Cell Biology Program, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada; Department of Oral Pathology and Oral Radiology, Institute of Dentistry, University of Turku, Turku, Finland.
Journal of Gastroenterology and Hepatology
|September 3, 2014
Summary
Inflammatory bowel disease (IBD) models showed oral microbiome changes, particularly in saliva. These findings link gut dysbiosis to oral health, suggesting implications for IBD management.
Area of Science:
- Microbiology
- Gastroenterology
- Oral Medicine
Background:
- Oral mucosal pathologies are common in inflammatory bowel disease (IBD).
- Host-microbiome interactions are crucial in IBD pathogenesis.
- The oral microbiome's role in IBD requires further investigation.
Purpose of the Study:
- To evaluate changes in the oral microbiome during colitis.
- To compare oral microbiome alterations in chemically induced colitis versus infection-induced colitis models.
Main Methods:
- Two mouse models of colitis were used: dextran sulfate sodium (DSS) and Citrobacter rodentium infection.
- Samples collected: tongue, buccal mucosa, saliva, colon, and stool.
- Bacterial 16S rRNA gene profiles analyzed using DGGE and qPCR for specific taxa.
Main Results:
- Both colitis models induced oral microbiome alterations, with saliva showing the most significant changes (up to 7.2%).
- DSS challenge resulted in greater oral microbiota changes than C. rodentium infection.
- Cluster analysis revealed substantial shifts: saliva (~35%), stool (~10%), tongue and buccal mucosa (~5%).
Conclusions:
- Colitis-associated dysbiosis is linked to altered oral cavity and saliva bacterial composition.
- Changes in the oral microbiota may play a role in the development and treatment of oral pathologies in IBD patients.
More Related Videos
Related Concept Videos
01:27Microbiota of the Large Intestine
90
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
90
01:30Development of Human Microbiota
59
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...
59
01:27Microbiota of the Stomach and Small Intestine
74
The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...
74
01:22Introduction to the Human Microbiota
146
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
146
01:18Functions of the Gut Microbiota
128
The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...
128