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Down-regulated expression of NPM1 in IMS-M2 cell line by (-)-epigallocatechin-3-gallate
Hoang Thanh Chi1, Bui Thi Kim Ly2, Hoang Anh Vu3
1Department of Molecular Cytogenetics, Hematology and Blood Transfusion Hospital in Ho Chi Minh City, Ho Chi Minh, Vietnam.
Objective:
To investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations.
Methods:
Cell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis.
Results:
EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells.
Conclusions:
The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.
