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Updated: Apr 24, 2026

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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
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Direct conversion of cytochrome c spectral shifts to fluorescence using photochromic FRET
Selen Manioglu1, Muge Atis, Mehdi Aas
1Department of Chemical and Biological Engineering, Koc University, Istanbul, 34450, Turkey.
Abstract:
Photochromic fluorescence resonance energy transfer (pcFRET) was used to monitor the redox activity of non-fluorescent heme protein. Venus fluorescent protein was used as a donor where its emission intensity was reversibly modulated by the absorption change of Cytochrome c.

