Listeria monocytogenes in foods. Isolation, characterization and control

The intent for examining foods for Listeria monocytogenes, i.e. for surveying, for epidemiological purposes, or to inspect consignments of foods for microbiological safety, determines which analytical method is to be used. For instance resuscitation of debilitated cells may be required, and the degree of accuracy and precision necessary should be considered. Moreover, in the case of acceptance-or-rejection monitoring target values for 'absence' of the pathogen have been amply but not always effectively discussed. Recommendations are given for assessing adequate repair of sublethally damaged populations of L. monocytogenes and for performance testing of selective enrichment and isolation media to be used for the isolation and enumeration of L. monocytogenes. An approach to the empirical assessment of reference values for L. monocytogenes in foods processed for safety is also presented. This relies on a data base of results obtained when examining foods manufactured and distributed according to practices previously validated by longitudinally integrated ('holistic') quantitative risk analysis.