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Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Study of a monoclonal antibody specific to epithelial cells of mammalian thymuses

P Parvaz1, C Auger, F Giraud

  • 1Laboratoire d'Immunologie UFR Alexis Carrel, Lyon, France.

Journal of Clinical & Laboratory Immunology
|November 1, 1989
PubMed

Hybridomas between spleen cells from BALB/c mice and Sp2/O-Ag14 mycloma cell line are performed by PEG fusion. An in vitro booster is realized with human thymic reticulo-epithelial cell cultures as antigen. A monoclonal antibody (3H9) directed against the thymic epithelium and stratified epithelia of mammals has been obtained and characterized.

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...