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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
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Surface free energy activated high-throughput cell sorting.

Xinru Zhang1, Qian Zhang, Tao Yan

  • 1Department of Mechanical Engineering and §Department of Civil and Environmental Engineering, University of Hawaii at Manoa , Honolulu, Hawaii 96822 United States.

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|September 4, 2014
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Summary
This summary is machine-generated.

A novel label-free bulk cell sorting method differentiates bacterial surface free energies (SFEs) for rapid, high-efficiency separation. This high-throughput technique sorts billions of cells quickly while maintaining high viability.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Clinical Research
  • Biophysics

Background:

  • Current cell sorting relies on single-cell analysis, limiting throughput.
  • Flow cytometry and microfluidics are common but can be time-consuming.
  • A need exists for rapid, high-capacity cell separation methods.

Purpose of the Study:

  • To develop and validate a label-free bulk cell sorting method.
  • To differentiate cells based on their surface free energies (SFEs).
  • To assess the efficiency, speed, and viability of the new sorting technique.

Main Methods:

  • A novel bulk cell sorting technique based on differentiating surface free energies (SFEs).
  • Demonstrated feasibility using model binary mixtures of bacterial species (e.g., Pseudomonas putida, Enterococcus faecalis, Salmonella Typhimurium, Escherichia coli).
  • Evaluated sorting efficiency, speed, cell viability, and enrichment of minor populations.

Main Results:

  • Successfully sorted large quantities (10^10) of bacterial cells within 30 minutes.
  • Achieved up to 96% sorting efficiency for individual species with a 99% cell viability ratio.
  • Demonstrated enrichment of minor cell populations (1%) to 99% purity.

Conclusions:

  • The SFE-activated method offers a label-free, high-throughput, low-cost, and simple approach to cell sorting.
  • This method can be used independently or as a prescreening tool for microbial discrimination.
  • Potential applications include sorting large cell quantities and abiotic particles in various scientific fields.