Related Experiment Video
Updated: Apr 24, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The complete mitochondrial genome of Papilio polytes (Lepidoptera Papilionidae)
Xiao-Wen Yang1, Li-Xia Hou1, Yu Zhang1
1a College of Life Science, Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University , Guilin , Guangxi , P.R. China.
Abstract:
The complete sequence mitochondrial genome of Papilio polytes was determined using long PCR and conserved primers walking approaches. The genome was 15,260 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of P. polytes were similar to other lepidopteran species. All protein-coding genes begin with ATG and ATT as initiation codon except COI using CGA. 8 genes (ATP8, ATP6, ND3, ND5, ND4L, ND6, Cytb and ND1) ended with TAA and TAG stop codon, the remaining five genes had incomplete stop codon T. The overall base composition of the genome in descending order was 39.51% A, 11.86% C, 40.75% T and 7.88% G, with a A + T bias of 80.26%. CR is located between the 12S rRNA and tRNA-Met genes and is 439 bp in length, with an AT content of 83.37%.
Related Concept Videos
Animal Mitochondrial Genetics
Polytene Chromosomes
Polytene Chromosomes
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Export of Mitochondrial and Chloroplast Genes
Genomic DNA in Eukaryotes

