Related Experiment Video

Updated: Apr 24, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

20.8K

Patents in therapeutic recombinant protein production using mammalian cells

Virginia Picanco-Castro, Marcela Cristina Correa de Freitas, Aline de Sousa Bomfim

  • 1FCFRP-USP, DACTB, Av do Cafe s/n, Monte Alegre, CEP 14040-903, Ribeirao Preto, SP, Brazil. elisa@fcfrp.usp.br.

Recent Patents on Biotechnology
|September 5, 2014
PubMed

More Related Videos

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization07:08

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

12.3K
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

30.2K

Related Concept Videos

Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
90
Recombinant DNA01:09

Recombinant DNA

Overview
92.2K
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
95