Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

12.5K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
12.5K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

2.2K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.2K
Photosystems01:32

Photosystems

7.0K
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
7.0K
DNA Packaging00:58

DNA Packaging

94.4K
Overview
94.4K
Structure of Porins01:21

Structure of Porins

3.0K
Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a  motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
3.0K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

6.1K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A hierarchical, compact and efficient phenanthrene supramolecular polymer light harvesting antenna.

Journal of materials chemistry. C·2026
Same author

Front Cover: First Access to Tetraazadiindenopyrenes via Selective Pyrrole Cyclization of Phenyl-Substituted Tetraazapyrene Derivatives on Au(111) (Small Sci. 5/2026).

Small science·2026
Same author

First Access to Tetraazadiindenopyrenes via Selective Pyrrole Cyclization of Phenyl-Substituted Tetraazapyrene Derivatives on Au(111).

Small science·2026
Same author

Connectivity Effect on Electronic Properties of Azulene-Tetraazapyrene Triads.

Molecules (Basel, Switzerland)·2026
Same author

Toward Gate-Tunable Topological Superconductivity in a Supramolecular Electron Spin Lattice.

Nano letters·2025
Same author

Discharge and electron correlation of radical molecules in a supramolecular assembly on superconducting Pb(111).

Nanoscale horizons·2025

Related Experiment Video

Updated: Apr 24, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.3K

Assembling multiporphyrin stacks inside the DNA double helix.

Mykhailo Vybornyi1, Alina L Nussbaumer, Simon M Langenegger

  • 1Department of Chemistry and Biochemistry, University of Bern , Freiestrasse 3, CH-3012 Bern, Switzerland.

Bioconjugate Chemistry
|September 5, 2014
PubMed
Summary

DNA successfully accommodates stacked porphyrins within double-stranded structures, forming H-aggregates with strong coupling and fluorescence quenching. This demonstrates DNA

More Related Videos

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

3.5K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

10.9K

Related Experiment Videos

Last Updated: Apr 24, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.3K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

3.5K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

10.9K

Area of Science:

  • Supramolecular Chemistry
  • Biotechnology
  • Materials Science

Background:

  • Porphyrins are versatile molecules with applications in various fields.
  • Controlled self-assembly of porphyrins is crucial for developing advanced materials.
  • DNA's structural properties offer a unique scaffold for molecular organization.

Purpose of the Study:

  • To investigate the incorporation and self-assembly of porphyrins within DNA duplexes.
  • To explore the formation of porphyrin H-aggregates inside a DNA scaffold.
  • To assess the impact of porphyrin stacking on DNA structure and stability.

Main Methods:

  • Synthesis of non-nucleosidic porphyrin building blocks.
  • Incorporation of porphyrins into complementary oligonucleotide strands.
  • DNA hybridization to form double-stranded structures.
  • Spectroscopic analysis (UV/vis, fluorescence, CD) to characterize porphyrin aggregation and DNA integrity.

Main Results:

  • Successfully formed DNA duplexes containing up to four face-to-face stacked porphyrins.
  • Porphyrins were accommodated within the DNA scaffold without disrupting the B-DNA structure.
  • Evidence of porphyrin H-aggregate formation and strong interstrand excitonic coupling.
  • Observed fluorescence quenching and significant circular dichroism (CD) effects, particularly in four-porphyrin stacks.

Conclusions:

  • DNA serves as a valuable template for the controlled assembly of porphyrin nanostructures.
  • The DNA scaffold enables the formation of molecularly defined porphyrin aggregates with tunable properties.
  • This approach opens avenues for designing novel functional materials based on DNA-porphyrin hybrids.