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Published on: September 15, 2017
Protective effect of kaempferol on LPS plus ATP-induced inflammatory response in cardiac fibroblasts
Xi-Lan Tang1, Jian-Xun Liu, Wei Dong
1Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
Insights
Kaempferol, a flavonoid, reduces inflammation in cardiac fibroblasts by inhibiting key pathways. This suggests its potential as a therapeutic agent for cardiovascular diseases.
Area of Science:
- Cardiovascular Biology
- Inflammation Research
- Pharmacology
Background:
- Cardiac fibroblasts are key players in cardiovascular disease pathogenesis and inflammation.
- Kaempferol, a flavonoid, exhibits anti-inflammatory properties with potential therapeutic applications.
- The effect of kaempferol on cardiac fibroblast inflammation remains unexplored.
Purpose of the Study:
- To investigate the anti-inflammatory effects of kaempferol on cardiac fibroblasts.
- To elucidate the underlying molecular mechanisms of kaempferol's action.
Main Methods:
- Cardiac fibroblasts were stimulated with lipopolysaccharide (LPS) plus ATP.
- The impact of varying kaempferol concentrations (12.5 and 25 μg/mL) was assessed.
- Key inflammatory markers (TNF-α, IL-1β, IL-6, IL-18) and signaling pathways (NF-κB, Akt) were analyzed.
Main Results:
- Kaempferol significantly suppressed the release of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-18.
- Kaempferol inhibited the activation of NF-κB and Akt signaling pathways.
- These effects were observed at kaempferol concentrations of 12.5 and 25 μg/mL.
Conclusions:
- Kaempferol demonstrates significant anti-inflammatory effects in LPS plus ATP-induced cardiac fibroblasts.
- Kaempferol attenuates cardiac fibroblast inflammation by suppressing NF-κB and Akt activation.
- Kaempferol holds promise as a potential therapeutic agent for cardiovascular inflammatory conditions.
Abstract:
Inflammatory response is an important mechanism in the pathogenesis of cardiovascular diseases. Cardiac fibroblasts play a crucial role in cardiac inflammation and might become a potential therapeutic target in cardiovascular diseases. Kaempferol, a flavonoid commonly existing in many edible fruits, vegetables, and Chinese herbs, is well known to possess anti-inflammatory property and thus has a therapeutic potential for the treatment of inflammatory diseases. To date, the effect of kaempferol on cardiac fibroblasts inflammation is unknown. In this study, we investigated the anti-inflammatory effect of kaempferol on lipopolysaccharide (LPS) plus ATP-induced cardiac fibroblasts and explored the underlying mechanisms. Our results showed that kaempferol at concentrations of 12.5 and 25 μg/mL significantly suppressed the release of TNF-α, IL-1β, IL-6, and IL-18 and inhibited activation of NF-κB and Akt in LPS plus ATP-induced cardiac fibroblasts. These findings suggest that kaempferol attenuates cardiac fibroblast inflammation through suppression of activation of NF-κB and Akt.
