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Updated: Apr 24, 2026

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
Published on: December 9, 2022
Comment on "Local impermeant anions establish the neuronal chloride concentration"
Heiko J Luhmann1, Sergei Kirischuk2, Werner Kilb2
1Institute of Physiology, University Medical Center, University of Mainz, Duesbergweg 6, D-55128 Mainz, Germany. luhmann@uni-mainz.de.
Abstract:
Glykys et al. (Reports, 7 February 2014, p. 670) proposed that cytoplasmic impermeant anions and polyanionic extracellular matrix glycoproteins establish the local neuronal intracellular chloride concentration, [Cl(-)]i, and thereby the polarity of γ-aminobutyric acid type A (GABAA) receptor signaling. The experimental procedures and results in this study are insufficient to support these conclusions. Contradictory results previously published by these authors and other laboratories are not referred to.
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