Related Experiment Videos
[Property and function of cell surface glycosyltransferases]
1Department of Biochemistry, University of Tokyo, Japan.
Human Cell
|March 1, 1989
Summary
Cell surface glycosyltransferases, though enzymatically similar to intracellular forms, mediate cell interactions by binding substrates without catalysis. Differences in protein chemistry and expression exist between surface and intracellular enzymes.
Area of Science:
- Cell Biology
- Glycobiology
- Biochemistry
Context:
- Glycosyltransferases were identified on mammalian cell surfaces in the 1970s.
- These surface enzymes share catalytic properties with intracellular forms but exhibit distinct characteristics.
- Monoclonal antibodies reveal differences in protein chemistry and expression levels during differentiation.
Purpose:
- To investigate the distinct roles and characteristics of cell surface glycosyltransferases.
- To understand the mechanism by which these enzymes mediate cellular interactions.
Summary:
- Cell surface glycosyltransferases are functionally distinct from intracellular enzymes.
- They engage in cell-cell and cell-matrix recognition and binding without enzymatic activity due to the lack of extracellular sugar donors.
- Differences in protein chemistry and expression patterns are observed, suggesting specialized functions.
Impact:
- Highlights the non-catalytic role of surface glycosyltransferases in cell adhesion and recognition.
- Provides insights into the complex mechanisms governing cell interactions and tissue organization.
- Suggests potential targets for therapeutic interventions in diseases involving cell adhesion.