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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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EPAS-1 mediates SP-1-dependent FBI-1 expression and regulates tumor cell survival and proliferation

Xiaogang Wang1, Peng Cao2, Zhiqing Li3

  • 1Department of Neurosurgery, Institute of Neurology, General Hospital of Shenyang Military Area Command, Shenyang 110016, China. xiaogangwangzy@163.com.

International Journal of Molecular Sciences
|September 6, 2014
PubMed

Insights

Endothelial PAS domain protein-1 (EPAS-1) is crucial for regulating Factor Binding IST-1 (FBI-1) induction. This discovery offers a potential new therapeutic target for various human cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Factor Binding IST-1 (FBI-1) is implicated in oncogenic transformation and tumorigenesis.
  • Over-expression of FBI-1 in human cancers highlights the need for understanding its regulatory mechanisms for therapeutic intervention.

Purpose of the Study:

  • To investigate the regulatory role of Endothelial PAS domain protein-1 (EPAS-1) in controlling FBI-1 expression.
  • To explore the potential of EPAS-1 as a novel cancer therapeutic target.

Main Methods:

  • Investigated the interaction between EPAS-1 and specificity protein-1 (SP-1), a known transcription factor for FBI-1.
  • Assessed the role of EPAS-1 in the SP-1-mediated induction of FBI-1.

Main Results:

  • EPAS-1 plays an essential role in the transcriptional induction of FBI-1 mediated by SP-1.
  • EPAS-1 was found to be involved in tumor cell survival and proliferation.

Conclusions:

  • EPAS-1 is a key regulator of FBI-1 induction.
  • EPAS-1 represents a promising novel therapeutic target for cancer treatment.

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