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Updated: Apr 24, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Data analysis for 16S microbial profiling from different benchtop sequencing platforms
Victor S Pylro1, Luiz Fernando W Roesch2, Daniel K Morais1
1Microbiology Department, Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil; AgroEcology Department, Rothamsted Research, Harpenden, Herts AL52JQ, United Kingdom.
Abstract:
Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied.
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