Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.1K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heparin combined with urokinase therapy for the treatment of acute portal vein thrombosis in children.

Pediatric surgery international·2026
Same author

Astragalin from Morus alba L. ameliorates high-fat diet-induced obesity-related metabolic disturbances in mice and is associated with hepatic AMPK/PI3K/AKT signaling.

Journal of ethnopharmacology·2026
Same author

Correction: A comparative study of phyllostachys edulis and its dwarf variant phyllostachys edulis 'Tubaeformis' at the anatomical, transcriptomic, and DNA methylation levels.

Frontiers in plant science·2026
Same author

UPLC Multiwavelength Quantification and PLS-DA Reveal Stir-Baking Degree-Dependent Marker Dynamics in Paeoniae Radix Alba.

Biomedical chromatography : BMC·2026
Same author

Reply to: Limited evidence of AI superiority in seasonal influenza vaccine strain selection.

Nature medicine·2026
Same author

A novel compound heterozygous variant in DNAH2: preliminary evidence of a potential genetic modifier for persistent cloaca in a Chinese family.

Pediatric surgery international·2026

Related Experiment Video

Updated: Apr 24, 2026

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

4.3K

CRISPR/Cas9 mediates efficient conditional mutagenesis in Drosophila.

Zhaoyu Xue1, Menghua Wu1, Kejia Wen1

  • 1School of Life Sciences, Tsinghua University, Beijing 100084, China.

G3 (Bethesda, Md.)
|September 7, 2014
PubMed
Summary

We developed a CRISPR/Cas9 system for precise gene editing in Drosophila, improving upon RNA interference (RNAi) efficiency for functional genomics. This method allows controlled gene inactivation in specific tissues and times.

Keywords:
CRISPR/Cas9DrosophilaGal4conditional mutagenesisgRNA

More Related Videos

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
08:19

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Published on: September 28, 2022

2.6K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.6K

Related Experiment Videos

Last Updated: Apr 24, 2026

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

4.3K
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
08:19

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Published on: September 28, 2022

2.6K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.6K

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Transgenic RNA interference (RNAi) is crucial for Drosophila functional genomics.
  • RNAi efficiency can be a limitation in gene silencing studies.

Purpose of the Study:

  • To develop a more efficient gene inactivation system in Drosophila.
  • To complement existing RNAi methods for functional genome studies.

Main Methods:

  • Developed a CRISPR/Cas9-mediated conditional mutagenesis system.
  • Combined tissue-specific Cas9 expression (Gal4/UAS) with ubiquitously expressed guide RNAs.
  • Utilized multiple guide RNAs targeting a single gene in transgenic vectors.

Main Results:

  • Achieved temporally and spatially controlled gene inactivation.
  • Demonstrated high efficiency of gene mutagenesis in specific tissues.
  • Successfully complemented RNAi approaches.

Conclusions:

  • CRISPR/Cas9 conditional mutagenesis is a simple and effective tool for Drosophila gene function analysis.
  • This system enhances the toolkit for genetic research by offering improved control and efficiency over RNAi.