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Enamel prism-like tissue regeneration using enamel matrix derivative.

Ying Cao1, May Lei Mei2, Quan-Li Li3

  • 1Faculty of Dentistry, The University of Hong Kong, Hong Kong, China; Stomatologic Hospital & College, Anhui Medical University, Key Lab. of Oral Diseases Research of Anhui Province, Hefei, China.

Journal of Dentistry
|September 7, 2014
PubMed
Summary

Enamel matrix derivative (EMD) promotes biomimetic remineralization of demineralized enamel, forming new enamel-like crystals. This finding suggests EMD could be a novel biomaterial for dental enamel repair.

Keywords:
Biomimetic mineralisationEmdogainEnamel matrix derivativeHydroxyapatiteRegeneration

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Area of Science:

  • Biomaterials Science
  • Dental Research
  • Mineralization Studies

Background:

  • Enamel matrix derivative (EMD) is known for periodontal regeneration.
  • Its role in biomimetic enamel mineralization is previously unreported.

Purpose of the Study:

  • To investigate the effect of EMD on biomimetic mineralization of demineralized enamel.
  • Utilized an in vitro agarose hydrogel model for enamel repair research.

Main Methods:

  • Demineralized human enamel slices were treated with EMD-calcium chloride (CaCl2) in an agarose hydrogel.
  • Incubation in a fluoride-containing phosphate solution.
  • Analysis using SEM, EDX, XRD, and nano-indentation for crystal formation and mechanical properties.

Main Results:

  • Enamel prism-like crystals with hexagonal apatite structures were observed.
  • EDX confirmed calcium, phosphorus, and fluorine presence.
  • XRD identified fluorinated hydroxyapatite, and nano-indentation showed increased elastic modulus and nanohardness.

Conclusions:

  • EMD effectively promoted in vitro biomimetic mineralization of demineralized enamel.
  • Facilitated the formation of enamel-like tissue, indicating potential for enamel repair.
  • This study pioneers the use of EMD in biomimetic mineralization for dental applications.