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Simple and reliable urea assay based on a signal accumulation type of ion-sensitive field-effect transistor
Naohiro Tomari1, Asako Kawasaki2, Yoshihiro Yamamoto1
1Kyoto Municipal Institute of Industrial Technology and Culture, 91 Chudouji Awata-cho, Shimogyo-ku, Kyoto 600-8813, Japan.
Journal of Bioscience and Bioengineering
|September 7, 2014
Abstract:
A simple urea assay was developed using a signal accumulation type of ion-sensitive field-effect transistor (SA-ISFET). Decreases in proton concentration resulting from urease-catalyzed hydrolysis of urea are detected by SA-ISFET as a change in potential. The method exhibits high sensitivity, linearity, and reproducibility when potential signals are accumulated 10-fold.

