From genes to protein mechanics on a chip
Related Experiment Video
Updated: Apr 24, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform
Published on: August 23, 2012
Marcus Otten1,2, Wolfgang Ott1,2, Markus A Jobst1,2
1Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.
Abstract:
Single-molecule force spectroscopy enables mechanical testing of individual proteins, but low experimental throughput limits the ability to screen constructs in parallel. We describe a microfluidic platform for on-chip expression, covalent surface attachment and measurement of single-molecule protein mechanical properties. A dockerin tag on each protein molecule allowed us to perform thousands of pulling cycles using a single cohesin-modified cantilever. The ability to synthesize and mechanically probe protein libraries enables high-throughput mechanical phenotyping.
More Related Videos
Related Concept Videos
Mechanical Protein Functions
From DNA to Protein
Mechanical Protein Function
Proteins: From Genes to Degradation
Transcription is the synthesis of RNA...
DNA Microarrays

