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Updated: Apr 24, 2026

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
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Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Published on: May 18, 2020

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[Molecular mechanism and related influence factors of Lin28/Let-7 axsis]

Lihua Rao, Xiaowen Huang, Sheng Xu

    Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Journal of Clinical Otorhinolaryngology Head and Neck Surgery
    |September 9, 2014
    PubMed

    Insights

    The Lin28/Let-7 axis, a key regulator in human tumors, involves Lin28 protein inhibiting Let-7 microRNAs (miRNAs) and promoting cancer. Understanding this axis is crucial for cancer research.

    Area of Science:

    • Molecular Biology
    • Oncology
    • Genetics

    Background:

    • The Let-7 microRNA (miRNA) family, comprising 13 members across nine loci, functions as a tumor suppressor.
    • Lin28 protein negatively regulates miRNA biogenesis by inhibiting Let-7 miRNA processing.
    • The Lin28/Let-7 axis plays a significant role in the development and progression of human tumors.

    Purpose of the Study:

    • To review the molecular mechanisms governing the Lin28/Let-7 axis.
    • To explore factors influencing the Lin28/Let-7 axis in the context of cancer.

    Main Methods:

    • Literature review of recent studies on the Lin28/Let-7 axis.
    • Analysis of molecular interactions between Lin28 and Let-7 miRNAs.
    • Investigation of regulatory feedback loops and oncogenic pathways.

    Main Results:

    • Lin28 selectively blocks Let-7 miRNA synthesis, thereby diminishing miRNA-mediated inhibition of proto-oncogenes.
    • Lin28 interacts with RNA helicases to concurrently enhance oncogene translation.
    • An incompletely understood mechanism shows Let-7 upregulation inhibits Lin28 expression and function.

    Conclusions:

    • The Lin28/Let-7 axis is a critical regulatory pathway in tumorigenesis.
    • Further research into the molecular intricacies of this axis may reveal novel therapeutic targets for cancer treatment.

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