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Cell wall bound anionic peroxidases from asparagus byproducts.
Sara Jaramillo-Carmona1, Sergio López, Sara Vazquez-Castilla
1Phytochemicals and Food Quality Group, Instituto de la Grasa (CSIC) , 41014 Seville, Spain.
Journal of Agricultural and Food Chemistry
|September 9, 2014
Summary
Asparagus byproducts yield cell wall bound peroxidases (POD) for wastewater bioremediation. Anionic asparagus peroxidase (AAP) effectively removes hydroxytyrosol, while cationic soluble peroxidases (CAP) target other phenols.
Area of Science:
- Biotechnology
- Environmental Science
- Biochemistry
Background:
- Asparagus byproducts contain valuable enzymes for bioremediation.
- Cationic soluble peroxidases (CAP) are known for phenol removal from wastewater.
- Cell wall bound peroxidases (POD) from asparagus also show potential.
Purpose of the Study:
- To purify and characterize cell wall bound peroxidases (POD) from asparagus byproducts.
- To compare the efficacy of different peroxidase fractions in removing specific phenolic compounds.
- To identify optimal peroxidase types for treating different types of phenol-contaminated wastewaters.
Main Methods:
- Purification of cell wall bound peroxidases using ion exchange and affinity chromatography.
- Isoelectric focusing to analyze isoenzyme composition of different POD fractions.
- In vitro detoxification assays using specific phenolic compounds (4-CP, 2,4-DCP, HT).
Main Results:
- Covalent forms constitute over 90% of cell wall bound POD.
- Covalent POD fractions are primarily anionic isoenzymes.
- Anionic asparagus peroxidase (AAP) demonstrated superior removal of hydroxytyrosol (HT).
- Cationic soluble peroxidases (CAP) were more effective for 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) removal.
Conclusions:
- Cell wall bound peroxidases, particularly AAP, are effective for specific phenol bioremediation.
- The choice of peroxidase depends on the target phenol contaminant.
- Asparagus byproducts offer a sustainable source for diverse peroxidase enzymes for wastewater treatment.
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